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M94A2759.TXT
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1994-10-25
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Document 2759
DOCN M94A2759
TI Diagnosis of HIV-1 infection with urine.
DT 9412
AU Hashinaka K; Hashida S; Saitoh A; Shinagawa H; Takamizawa A; Oka S;
Shimada K; Ishikawa E; Department of Biochemistry, Medical College of
Miyazaki, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):231 (abstract no. PB0354). Unique
Identifier : AIDSLINE ICA10/94369816
AB OBJECTIVE: To develop and test an enzyme immunoassay for diagnosis of
HIV-1 infection by detection of anti-HIV-1 IgG in urine. METHOD: An
ultrasensitive enzyme immunoassay (immune complex transfer enzyme
immunoassay) using recombinant reverse transcriptase (R-RT) as antigen
was developed. The immune complex consisting of 2,4-dinitrophenyl
(DNP)-bovine serum albumin-R-RT conjugate, anti-HIV-1 IgG and
R-RT-beta-D-galactosidase (GAL) conjugate was trapped onto anti-DNP
IgG-coated polystyrene balls (PS), eluted with DNP-L-lysine and
transferred to (anti-hIgG gamma-chain) IgG-coated PS. GAL activity was
assayed by fluorometry. RESULTS: Using 100 microliters of urine, the
lowest signals among 60 asymptomatic carriers, 11 patients with ARC and
12 patients with AIDS were 14-, 29- and 10-fold, respectively, higher
than the highest signal among 100 seronegatives. When urine was
concentrated, signals were proportionally enhanced for seropositives but
not significantly increased for seronegatives. Anti-HIV-1 IgG in urine
was stable at -20 degrees C for at least 6 months. CONCLUSION: From the
above results, the sensitivity and specificity were expected to be both
100% even for a larger number of cases, although the number of cases
tested was limited.
DE beta-Galactosidase/ANALYSIS Acquired Immunodeficiency Syndrome/URINE
AIDS-Related Complex/URINE Dinitrophenols Evaluation Studies Human
HIV Antibodies/*URINE HIV Infections/*DIAGNOSIS/URINE
HIV-1/*IMMUNOLOGY IgG/IMMUNOLOGY/*URINE *Immunoenzyme Techniques
Microspheres Recombinant Proteins/ANALYSIS/IMMUNOLOGY Reverse
Transcriptase/IMMUNOLOGY Sensitivity and Specificity Serum Albumin,
Bovine MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).